- 《英汉医学词典》recombinant clone
- 重组克隆
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recombinant clone中文什么意思
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- the pcr product was cloned into pmd18-t vector . the positive recombinant clone was identified by pcr and endonuclease digest
提取重组质粒经pcr鉴定和酶切鉴定后，对插入片段进行序列测定及分析。 - recombinant cloning vector of pgem-hc and pgem-ha were constructed by ligating the hc and ha genes into pgem t east vector and transformating into jm109.3
分别将目的基因hypoderminc和hypodermina与克隆载体(pgemteasy)连接并转化到宿主菌jm109中，构建了重组克隆载体pgem-hc和pgem-ha。 - then the linked products were transformed into the high competent cell of e . coli dh5a . based on-complementation of the detective-galactosidase, positive recombinant clone were screened from x-gal plate
从引物和基因序列的比对分析结果看，zhyf006序列与上下游引物的配对比例分别为s4 - in this experiment, ri gene was amplified by pcr method from recombinant cloning vector pt7-ri, which had been constructed by dr . yu xiu-ping in this laboratory and sequenced by takara biotechnology ( dalian ) co ., ltd .
于秀平博士已经构建了ri的克隆载体pt7-ri，测序表明与已知人胎盘ri基因的cdna序列完全相同。本文的工作是在此基础之上完成的。 - in this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il-2 gene . about 600 bp chicken il-2 cdna fragment was cloned from cona-stimulated chicken splenocytes by reverse transcription-polymerase chain reaction ( rt-pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next, we construct recombinant plasmid pproex ? t-il-2 . the cdna of chicken il-2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t-il-2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds-page and western blot that a 18kda protein which was equal to chicken il-2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il-2 protein
经酶切鉴定及dna序列测定，该基因为鸡il-2基因，其序列与sundick等报道的完全一致。在此基础上，我们把鸡il-2基因亚克隆到大肠杆菌原核表达载体pproex~(tm)ht中，构建重组表达质粒并进行确证性序列测定，重组质粒测序结果表明将编码鸡il-2成熟蛋白的基因正确地插入到原核表达载体pproex~(tm)ht的目的位点。重组质粒转化受体菌dh5后用iptg于37进行诱导培养，sds-page和westernblot分析显示，表达的鸡il-2融合蛋白分子量约为18kda，表达的融合蛋白经薄层扫描发现目的蛋白表达量约占菌体蛋白的30。
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